Sustained thrombolysis with DNA-recombinant tissue type plasminogen activator in rabbits.

In Vivo Thrombin Inhibition Enhances and Sustains Arterial Recanalization With Recombinant Tissue-tpe Plasminogen Activator

The effects of heparin and the synthetic competitive thrombin inhibitor (2R,4R)-4-methyl-1[NM-(3-methyl-1,2,3,4-tetrahydro-8-quinolinesulfonyl)-L-arginyl]-2-piperidinecarboxylic acid monohydrate (Argatroban) on thrombolysis with recombinant tissue-type plasminogen activator (rt-PA) was studied in groups of six or seven rabbits with arterial thrombosis. The model consisted of a whole-blood clot ...

Dual frequency ultrasound-enhanced tissue plasminogen activator thrombolysis in an in vitro human clot model

Introduction: Stroke causes death and disability in patients throughout the world. At present, the only FDA- approved drug for ischemic stroke is recombinant tissue plasminogen activator (rt- PA). Unfortunately, rtPA can cause intracerebral hemorrhage and must use within limited time window (within 3-4.5 hour after onset of stroke). Ultrasound with rtPA loaded liposomes (rtPA_L...

Optimizing refolding condition for recombinant tissue plasminogen activator

Low molecular size additives such as L-arginine and the redox compounds have been used both in the culturemedium and in vitro refolding to increase recombinant proteins production. Additives increase proteinrefolding and yield of active proteins by suppressing aggregate formation or enhancing refolding process.In this work, a comparative study was performed on refolding of rec...

Ultrasound-assisted thrombolysis for stroke therapy: better thrombus break-up with bubbles.

BACKGROUND AND PURPOSE Ultrasound has been shown to increase recombinant tissue plasminogen activator thrombolysis through stable cavitation, or sustained bubble activity, but this mechanism needs further optimization. Use of low-frequency ultrasound in combination with microbubbles stabilized against dissolution, in the form of ultrasound contrast agents, has resulted in greater lytic efficacy...

Author's response to reviews Left Ventricular Apical Thrombus After Systemic Thrombolysis with Recombinant Tissue Title: Plasminogen Activator in a Patient with Acute Ischemic Stroke Authors: